Agilent Technologies InfinityLab LC Series Manuale utente

Agilent Technologies

Agilent InfinityLab LC Series

1260 Infinity II

Fluorescence Detectors

User Manual

Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual

Notices

No part of this manual may be reproduced

in any form or by any means (including

electronic storage and retrieval or transla-

tion into a foreign language) without prior

agreement and written consent from Agi-

lent Technologies, Inc. as governed by

United States and international copyright

laws.

Manual Part Number

G7121-90000 Rev. B

Edition

08/2018

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connection

with the furnishing, use, or perfor-

mance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a

license and may be used or copied only in

accordance with the terms of such license.

Restricted Rights Legend

If software is for use in the performance of

a U.S. Government prime contract or sub-

contract, Software is delivered and licensed

as “Commercial computer software” as

defined in DFAR 252.227-7014 (June 1995),

or as a “commercial item” as defined in

FAR 2.101(a) or as “Restricted computer

software” as defined in FAR 52.227-19

(June 1987) or any equivalent agency regu-

lation or contract clause. Use, duplication

or disclosure of Software is subject to Agi-

lent Technologies’ standard commercial

license terms, and non-DOD Departments

and Agencies of the U.S. Government will

receive no greater than Restricted Rights as

defined in FAR 52.227-19(c)(1-2) (June

1987). U.S. Government users will receive

no greater than Limited Rights as defined in

FAR 52.227-14 (June 1987) or DFAR

252.227-7015 (b)(2) (November 1995), as

applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated condi-

tions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

performed or adhered to, could

result in personal injury or

death. Do not proceed beyond a

WARNING notice until the indi-

cated conditions are fully

understood and met.

Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual 3

In This Guide

This manual covers the Agilent InfinityLab LC Series Fluorescence Detectors:

•the Agilent 1260 Infinity II Fluorescence Detector (G7121A), and

•the Agilent 1260 Infinity II Fluorescence Detector Spectra (G7121B).

1 Introduction to the Fluorescence Detector

This chapter gives an introduction to the detector and instrument overview.

2 Site Requirements and Specifications

This chapter provides information on environmental requirements, physical

and performance specifications.

3 Using the Fluorescence Detector

This chapter explains the essential operational parameters of the module.

4 Preparing the Module

This chapter provides information on how to set up the module for an analysis

and explains the basic settings.

5 Optimizing the Detector

This chapter provides information on how to optimize the detector.

6 Troubleshooting and Diagnostics

Overview about the troubleshooting and diagnostic features.

7 Error Information

This chapter describes the meaning of error messages, and provides

information on probable causes and suggested actions how to recover from

error conditions.

4 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual

In This Guide

8 Test Functions and Calibration

This chapter describes the tests for the module.

9 Maintenance

This chapter provides general information on maintenance of the detector.

10 Parts for Maintenance and Repair

This chapter provides information on parts for maintenance and repair.

11 Identifying Cables

This chapter provides information on cables used with the Agilent InfinityLab

LC Series modules.

12 Hardware Information

This chapter describes the detector in more detail on hardware and

electronics.

13 Appendix

This chapter provides safetey and other general information.

Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual 5

Contents

1 Introduction to the Fluorescence Detector 9

Introduction to the Detector 10

How the Detector Operates 15

Raman Effect 18

Optical Unit 19

Analytical Information From Primary Data 27

System Overview 32

Bio-inert Materials 38

2 Site Requirements and Specifications 41

Site Requirements 42

Physical Specifications 45

Performance Specifications 46

3 Using the Fluorescence Detector 53

Magnets 54

Turn on/off 55

Status Indicators 57

Instrument Configuration 58

Set up the Detector with Agilent Open Lab ChemStation 60

The Detector User Interface 61

Detector Control Settings 63

Method Parameter Settings 64

Advanced Settings 65

Acquire Spectra (G7121B Only) 68

Special Settings 69

Time Table 72

Instrument Curves 73

Agilent Local Control Modules 74

6 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual

Contents

4 Preparing the Module 77

Leak and Waste Handling 78

Before You Start 80

Solvent Information 81

5 Optimizing the Detector 87

Getting Started and Checkout 88

Method Development 93

Example: Optimization for Multiple Compounds 108

How to collect spectra with modes SPECTRA ALL IN PEAK and APEX SPECTRA

ONLY 116

Optimization Overview 119

Design Features Help Optimization 121

Finding the Best Wavelengths 122

Finding the Best Signal Amplification 124

Changing the Xenon Flash Lamp Frequency 130

Selecting the Best Response Time 132

Reducing Stray Light 135

6 Troubleshooting and Diagnostics 137

Available Tests vs User Interfaces 138

Agilent Lab Advisor Software 140

Diagnostic Signals 141

Monitoring of Additional Signals 144

7 Error Information 147

What Are Error Messages 148

General Error Messages 149

Detector Error Messages 156

8 Test Functions and Calibration 165

Introduction 166

Diagram of Light Path 170

Lamp Intensity Test 171

Raman ASTM Signal-to-Noise Test 173

Wavelength Accuracy Test 178

Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual 7

Contents

Wavelength Verification and Calibration 182

Wavelength Calibration Process 184

Wavelength Calibration Procedure 187

Excitation and Emission Grating Resistance History 191

D/A Converter (DAC) Test 192

Dark-Current Test 194

Using the Built-in Test Chromatogram 196

Other Lab Advisor Functions 198

9 Maintenance 205

Introduction to Maintenance 206

Warnings and Cautions 207

Overview of Maintenance 209

Cleaning the Module 210

Remove and Install Doors 211

Exchanging a Flow Cell 213

How to use the Cuvette 217

Flow Cell Flushing 218

Correcting Leaks 219

Replace Leak Handling System Parts 221

Replacing Module Firmware 223

Tests and Calibrations 224

10 Parts for Maintenance and Repair 225

Overview of Maintenance Parts 226

Kits 228

11 Identifying Cables 231

Cable Overview 232

Analog Cables 234

Remote Cables 236

CAN/LAN Cables 240

Agilent Module to PC 241

USB Cables 242

8 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual

Contents

12 Hardware Information 243

Firmware Description 244

Electrical Connections 247

Interfaces 250

Setting the 6-bit Configuration Switch 258

Instrument Layout 262

Early Maintenance Feedback 263

13 Appendix 265

General Safety Information 266

Waste Electrical and Electronic Equipment (WEEE) Directive 272

Radio Interference 273

Sound Emission 274

Solvent Information 275

Agilent Technologies on Internet 276

Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual

Agilent Technologies

Introduction to the Fluorescence

Detector

Introduction to the Detector 10

Fluorescence Detector (FLD) 11

Fluorescence Detector (FLD) Spectra 13

How the Detector Operates 15

Raman Effect 18

Optical Unit 19

Reference System 26

Analytical Information From Primary Data 27

Fluorescence Detection 27

Phosphorescence Detection 28

Processing of Raw Data 29

System Overview 32

Operating Principle 32

Leak and Waste Handling 33

Bio-inert Materials 38

This chapter gives an introduction to the detector and instrument overview.

10 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual

Introduction to the Fluorescence Detector

Introduction to the Detector

Table 1 Detector versions

Version Description

G7121A Introduced as 1260 Infinity II FLD without

spectra and multi-signal capabilities. Maximum

data rate is 74 Hz.

G7121B SPECTRA Introduced as 1260 Infinity II FLD with spectra

and multi-signal capabilities. Maximum data

rate is 148 Hz. The G7121B can be converted to

G7121A (emulation mode).

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