Optika Italy B-800 Series Manuale utente
Version: 2
Issued: 03, 08, 2015
Model
B-800BF
B-800PH
B-800 Series
INSTRUCTION MANUAL
Page 2
Table of Contents
Warning
Symbols and conventions
Safety Information
Intended use
List of accessories and spare parts
Overview
Unpacking
Assembling
Using the microscope
Maintenance
Troubleshooting
Equipment disposal
Page 3
Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-
nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this
manual contains important information on safety and maintenance, and that it must therefore be made acces-
sible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does
not comply with this manual.
Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-
ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
Page 4
List of accessories and spare parts
CAT. NO. DESCRIPTION
M-1004.N Centering telescope, 30mm diameter
M-005 Micrometric slide, 26x76mm, range 1mm, div. 0,01mm
M-613 Polarizing set (lters only).
M-615 Lambda lter for polarizing set.
M-617.1N Phase contrast set with IOS PLAN objective 40x.
M-977 Green filter, 45mm diameter
M-690 Eyecup (pair).
M-619 Photo tube adapter for full frame SLR camera.
M-173 Photo adapter for APS-C and Full Frame Reex cameras.
M-620 C-Mount adapter for 1/3” sensor.
M-620.1 C-Mount adapter for 1/2” sensor.
M-699 Universal adapter for OPTIKAM & DIGI cameras (only models with eyepiece adapter,
23mm).
M-114 C-Mount adapter for 1/2” sensor.
M-116 C-Mount adapter for 2/3” sensor.
M-113.1 Ring adapter, 30mm (for monocular and binocular microscopes).
15008 OPTIKA immersion oil, 10ml.
Page 5
Overview
COARSE
FOCUSING
KNOB
NOSEPIECE
DIOPTRIC
ADJUSTMENT
RING
PHOTO PORT
MAIN ON/OFF
EYEPIECE
CONDENSER
CENTERING
KNOBS
APERTURE
DIAPHRAGM
FIELD DIAPHRAGM
BRIGHTNESS
ADJUSTMENT KNOB
FOCUS-STOP KNOB
FINE FOCUSING
KNOB
OBJECTIVE
STAGE
Page 6
Put the microscope stand on a
solid table. Insert the optical
head above the attachment,
using the 2mm Allen wrench
to tighten the screw. (Fig.1)
Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Assembling
Once you open the box, these are the microscope’s components:
① Condenser
② Optical head
③ Main body
④ Eyepieces
⑤ Power supply
⑥ Stage
⑦ Objectives
①
②
③
④
⑤
⑥
⑦
Fig.1
Page 7
Insert the condenser under
the stage: position until it is
well inserted into its holder
(under the condenser there is
a pin that must fully enter the
guide of the holder). (Fig. 3)
AFTER INSERTING THE
CONDENSER, TIGHTEN
THIS SCREW
Insert both eyepieces into
the tubes of the optical head.
(Fig.2)
Mount the stage: lower the
support using the coarse
focus knob, then place the
stage and rmly tighten the
lock screw. (Fig.4)
Fig.2
Fig.3
Fig.4
Screw each objective into the
thread of the nosepiece, in
order of magnication. (Fig.5)
Fig.5
Page 8
Insert the power supply jack
on the connector at the rear.
(Fig.6)
Fig.6
In order to activate the transmitted light illu-
minator, insert the plug of the external power
supply into the socket and turn on the main
switch on the side of the main body. Turn the
brightness adjustment knob to a brightness
suitable for observation. (Fig.7)
Loosen the lock-screw, turn
the observation head to
a comfortable position for
observation, and then lock the
lock-screw. (Fig.8)
LED settings
Adjust the observation
head
MAIN
SWITCH
Using the microscope
Fig.7
Fig.8
Page 9
Hold the right and left parts of
the observation head using
both hands and adjust the
interpupillary distance by
turning the two parts until one
circle of light can be seen.
(Fig.9)
Adjust interpupillary
distance
Fig.9
Place the specimen on
the stage
Focus tension
adjustment
Fix the specimen slide on the
mechanical stage using the
slide-clamp. Ensure that the
specimen is centred over the
stage opening. (Fig.10)
Turn the tension-adjust knob
to get a suitable tension for
the focus system. (Fig.11)
NOTE: if the tension is too lo-
ose, the stage could go lower
by itself or the focus easily
lost after ne adjustment. In
this case, rotate the knob in
order to increase the friction.
Fig.10
Fig.11
Focus-stop knob
Loosen the focus-stop knob,
rotate the coarse focusing
knob to bring the slide into
focus with a 4X or 10X objecti-
ve. Then rotate the focus-stop
in order to block the height of
the stage. This simplies the
next focusing operations.
The focus-stop knob is also
useful to avoid accidental con-
tacts between objective and
specimen. (Fig.12) Fig.12
Page 10
Centering the
condenser
Diopter adjustment
Insert the swing-out lens of
the condenser ① and fully clo-
se the eld diaphragm ②. Ro-
tate the condenser adjustment
knob ③ until you see a sharp
image of the closed eld
diaphragm (a bright spot of
light). Act on the condenser
centering screws ④ until you
move the bright spot in the
center of the image eld. Then
re-open the eld diaphragm
②. (Fig.14)
Adjust the aperture of the iris
diaphragm under the conden-
ser to set the numerical aper-
ture of the illuminator, thus
controlling image contrast and
resolution. It is suggested to
set the aperture diaphragm
between 70% and 80% of
objective’s N.A. (Fig.15)
Adjust the ne focusing knob
to get the image sharp and
clear while observing with
your right eye, then turn the
left diopter ring to a sharp
and clear image also with the
other eye. The highpoint eye-
pieces allow the user to wear
glasses. (Fig.13)
NOTE :For the optimal para-
focality of the image, it’s sug-
gested to wear your glasses
during the normal use of the
microscope. Fig.13
APERTURE IRIS
DIAPHRAGM IMAGE
CENTERING THE CONDENSER
OBJECTIVE PUPIL
30-20%
70-80%
Fig.15
①
③
④
②
Fig.14
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