Optika Italy B-383 Series Manuale utente

Ver. 2.1 2022

INSTRUCTION MANUAL

B-383 Series

Model

B-383MET

Page 2

Table of Contents

1. Warning 3

2. Safety Information 3

3. Package content 4

4. Unpacking 5

5. Intended use 5

6. Symbols and conventions 5

7. Instrument description 6

8. Assembling 8

9. Summary of transmitted light observation procedures 10

10. Use of the microscope in transmitted light 11

10.1 Turning on the instrument 11

10.2 Light intensity adjustment 11

10.3 Adjusting the interpupillary distance 11

10.4

Diopter adjustment 11

10.5 Coarse focus tension adjustment 12

10.6

Coarse upper limit lever 12

10.7 Stage 12

10.7.1 Use of the glass plate 12

10.8 Centering the condenser 13

10.9 Aperture diaphragm 13

11. Summary of reflected light observation procedures 14

12. Use of the microscope in reflected light 15

12.1 Turning on the instrument 15

12.2 Light intensity adjustment 15

12.3 Useofelddiaphragm(FS) 15

12.4 E󰀨ectsofelddiaphragm 15

12.5

Useofaperturediaphragm(AS) 16

12.6 Useofcolorlters 16

12.7 Use of polarized light 16

13. Microphotography 18

13.1 Use of C-mount cameras 18

13.2 Useofreexcameras 18

14. Maintenance 19

15. Troubleshooting 20

Equipment disposal 22

Page 3

1. Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It

is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains

important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.

We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.

2. Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage

of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The

equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please

follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

Page 4

3. Package content

① Microscopeframe

② ReectedlightIlluminator

③ Observationhead

④ Eyepieces

⑤ Objectives

⑥ Tensionadjustmenttool

⑦ Allenwrench

⑧ Colorlters

⑨ Analyzer+polarizer

⑩ Glassplate

⑪ Dustcover

⑫ Powersupply

①

③

④

⑤

⑥

⑦

⑧

②

⑨⑩

⑪

⑫

Page 5

4. Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the

top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-

aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it

on a stable desk.

Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other

residuals may deteriorate the nal image quality and corrode the optics surface in a short time.

5. Intended use

Standard models

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

IVD Models

Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.

6. Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with caution.

ELECTRICAL SHOCK

This symbol indicates a risk of electrical shock.

Page 6

PHOTO/TV PORT

DIOPTER

ADJUSTMENT RING

OBSERVATION

HEAD

7. Instrument description

SLIDE HOLDER

ANALYZER

EYEPIECES

CONDENSER

STAGE

REVOLVER

OBJECTIVES

X/Y MOVEMENT

KNOBS

CONDENSER

CENTERING

SCREWS

APERTURE

DIAPHRAGM

LED FOCUS LEVER

POLARIZER

TENSION

ADJUSTMENT

RING

Page 7

BRIGHTNESS ADJUSTMENT

KNOB

REFLECTED LIGHT

ILLUMINATOR

FIELD DIAPHRAGM

(FS)

APERTURE

DIAPHRAGM (AS)

Opposite side

FOCUS LOCK LEVER

CONDENSER HEIGHT

ADJUSTMENT KNOB

FINE FOCUS KNOB

COARSE FOCUS KNOB

LED HOUSING

Page 8

8. Assembling

1. Insert the reected light illuminator ① on the frame and lock

the locking knob ② with the provided Allen wrench. (Fig. 1)

2. Connect the illuminator cable ③ to the socket ④placed on

the back side of the frame. (Fig. 2)

3. Insert the optical head above the epi-illuminator and tighten

the locking screw. (Fig. 3)

• Hold the head with one hand during the locking in order

to avoid that the head falls.

FFig. 1ig. 1

FFig. 2ig. 2

FFig. 4ig. 4

FFig. 3ig. 3

①

②

③

④

4. Insert both eyepieces into the tubes of the optical head. (Fig.

Page 9

5. Insert the polarizer. (Fig. 5)

6. Remove the protection cap and insert analyzer. (Fig. 6-7)

7. Insert the power supply jack in the connector placed on the

back side of the frame. (Fig. 8)

FFig. 5ig. 5

FFig. 6ig. 6

FFig. 7ig. 7

FFig. 8ig. 8

Page 10

9. Summary of transmitted light observation procedures

(Used commands) (Chapter)

Main switch

Nosepiece

Coarse and fine focus knobs

Observation head

Diopter adjustment ring

Aperture diaphragm

Nosepiece

Brightness adjustment knob

Coarse and fine focus knobs

Put on “I” the main switch and adjust brightness

Insert 10x objective into the light path

Focus the specimen

Begin observation

Adjust aperture diaphragm

Insert the desired objective into the light path and focus the

specimen

Adjust interpupillary distance

Adjust diopter

Adjust brightness

Slide holder

X/Y movement knobs

Place a specimen on the stage 10

Brightness adjustment knob 10

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