Optika Italy IM-5FLD Manuale utente

Version: 1

Issued: 22, 03, 2018

Model

IM-5FLD

IM-5 Series

INSTRUCTION MANUAL

Page 2

Table of Contents

1. Warning

2. Symbols and conventions

3. Safety Information

4. Intended use

5. Overview

6. Unpacking

7. Assembling

8. Using the microscope

9. Transmitted Light Observation modes

10. Fluorescence Observation mode

11. Simultaneous Phase Contrast + Fluorescence Observation

12. Maintenance

13. Troubleshooting

Equipment disposal

Page 3

1. Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of

maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you

that this manual contains important information on safety and maintenance, and that it must therefore be made

accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses

that does not comply with this manual.

2. Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with caution.

ELECTRICAL SHOCK

This symbol indicates a risk of electrical shock.

3. Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the

operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety

regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility

to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure

safe operation of the unit.

4. Intended use

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Page 4

5. Overview

FIELD STOP

LEVER

PHASE

CONTRAST SLIDER

TRINOCULAR

VIEWING TUBE

(FIXED)

DIOPTRIC

ADJUSTMENT

RING

EYEPIECE

LED

HOUSING

CONDENSER

COARSE

FOCUS KNOB

FINE FOCUS KNOB

TENSION

ADJUSTMENT

COLLAR

OBJECTIVE

STAGE

MICROSCOPE

BODY

LIGHT PATH

SELECTOR

LEVER

STAGE PLATE

LED CENTERING

KNOBS

X-Y STAGE

MOVEMENT

KNOBS

ECO BUTTON

TRANSMITTED

LIGHT INTENSITY

INDICATOR

CONDENSER

CENTERING

SCREWS

APERTURE

STOP LEVER

BRIGHTNESS

ADJUSTMENT

KNOB

(TRANSMITTED

LIGHT)

MAIN

SWITCH

Page 5

5. Overview (opposite side)

FLUORESCENCE LED

LAMP HOUSING

FLUORESCENCE

FILTER SELECTOR

REFLECTED LIGHT

DIAL

FLUORESCENCE

UV PROTECTION

SHIELD

FLUORESCENCE

FILTER INDICATOR

REFLECTED

LIGHT INTENSITY

INDICATOR

DIFFUSION

FILTER

Page 6

6. Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container

and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)

fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope

from the container and put it on a stable desk.

7. Assembling

Once you open the box, these are the microscope’s components:

① Microscope body

② Condenser

③ Stage extension

④ Mechanical stage

⑤ Phase contrast slider

⑥ Darkeld slider

⑦ Eyepieces

⑧ Centering telescope

⑨ Metal insert for stage

⑩ Glass insert for stage

⑪ Centering screws

⑫ Power supply

⑬ Power cord

⑭ Green lter (IF550)

⑮ Blue lter (LBD)

⑯ Dust cover

⑰ Holder for Petri diam. 38 mm

⑱ Holder for Terasaki and Petri diam. 65 mm

⑲ Holder for slide and Petri diam. 54 mm

⑳ Lens cleaning tissues

㉑Allen screwdriver

㉒UV protective shield

㉓ Fluorescence lter selector

㉔ Diffusion lter

㉕Lamp housing

⑪

⑩③

⑤

⑥

⑨

①

②

④

⑦

⑧

⑫

⑬

⑭

⑮

⑯

⑰

⑱⑲

⑳㉑㉒

㉓

㉔

㉕

Page 7

③

Fig.2

Fig.3

Fig.4

Fig.5

Fig.1

1. Turn the coarse focusing knob ① until the

nosepiece reaches its lowest position.

► For a safe transport, the nosepiece is

placed in the lowest position and the

tension adjustment collar ②is adjust-

ed to the appropriate tension when the

microscope leaves the factory. (Fig.1)

2. Screw the lowest magnication objective

on to the nosepiece from the right side,

then turn the turret clockwise. Install the

other objectives in the same way, following

the sequence from low to high magnica-

tion.

► Note: the objectives can also be in-

stalled through the stage opening.

(Fig.2)

► Clean the objectives regularly. In inverted

microscopes, the objectives are very sen-

sitive to dust.

► To prevent dust and contamination from

entering the microscope, cover all the un-

used holes with dust caps ③. (Fig.3)

► When operating, use the low magnication

objective (4x or 10X) to search and focus

the specimen, then switch to higher magni-

cations.

► When switching between objectives, slowly

turn the nosepiece until it clicks. The click

means that the objective is in the right posi-

tion, in the center of the light path.

①

②

7.1 Installing the objectives

The stage extension can be installed on either

side of the stage to enlarge the working sur-

face. The mechanical stage must be installed

on the right side of the microscope.

1. Installing the stage extension: screw the

bolts on to the extension, then mount the

extension from below the stage. (Fig.4)

2. Installing the mechanical stage: as for the

extension, the mechanical stage is xed

with two bolts under the stage. (Fig.5)

7.2 Installing the stage extension

and the mechanical stage

Page 8

1. When using the stage inserts, make sure

that the insert is horizontal.

2. Install the stage insert in the stage open-

ing. (Fig.6)

7.3 Installing the stage insert

7.4 Installing the eyepieces

Insert both eyepieces into the tubes of the

optical head. (Fig.7)

Fig.6

Fig.7

Fig.8

1. Slide the condenser into the condenser

holder, inserting the pin on the rear side of

the condenser with the alignment groove

in the condenser holder. (Fig. 8)

2. Lock the condenser with the locking knob

① on the right side of the condenser hol-

der. (Fig. 9)

7.5 Installing the condenser

Fig.9

①

1. Turn the main switch ① to “O”(o󰀨) before

connecting the power cord. (Fig.10)

2. Insert the plug of the provided power

supply into the power socket of the

microscope. (Fig.11)

3. Plug the power cord int the socket of the

power supply (Fig.12)

4. Plug the power cord into the mains socket.

Check for a safe connection.

► Please use the supplied power cord.

If lost or damaged, please refer to

qualied service.

► Connect the power cord to a grounded

power supply only.

7.6 Connecting the power cord

Fig.10

Fig.11 Fig.12

①

Page 9

1. Unscrew the lamp housing lock (Fig. 13)

2. Insert the lamp housing in the

uorescence illuminator connector in the

back side of the microscope. (Fig.14)

3. Tighten the locking screw using the

provided allen screwdriver. (Fig.15)

4. Connect the cable of the lamp hoising

to the connector in the back side of the

microscope. (Fig.16)

7.7 Installing the lamp housing

Fig.13

Fig.14

Fig.15

Fig.16

Page 10

Turning on the light source

Connect the power, turn on the main switch ①.

(Fig.17)

Adjusting the brightness

Turn the brightness adjustment knob ②to

increase and decrease the brightness. (Fig.18)

While rotating the brightness adjustment knob,

the LED indicator on the front panel ③ will turn

ON or OFF some segments. (Fig.19)

Adjusting the tension

► The coarse focusing knob ①is pre

adjusted to a tight tension upon

leaving the factory.

If the nosepiece drops down by itself, or the

specimen defocuses while adjusting the ne

focus knob ③, the coarse focus knob ①is too

loose. Turning the tension adjustment collar

② in clockwise direction tightens the coarse

focus tension. Rotate in the opposite direction

to decrease the tension. (Fig.20)

Setting the specimen

► For the best image quality, use asks,

Petri dishes and slides with a 1.2 mm

thickness.

1. Place the proper insert for your specimen

(according to the table on the right) on the

stage, and x it with the stage clip.

2. Turning the X and Y knobs, move the

specimen to the required position. (Move-

ment Range: 120 (width) × 78 (length)

mm).

Moving the specimen

Move the specimen to the desired position by

freehand or by turning the knobs of the me-

chanical stage.

► When switching objectives, take care

not to touch the adaptor plates with

the objectives, as their weight may

damage the front lens.

8.1 Initial setup

8.2 Stage

8. Using the microscope

Fig.17

Fig.18

①

②

①

②

③

Fig.20

Fig.19

Holder for Petri diameter 38 mm (holder for Terasaki

needed) (provided with the microscope)

Holder for Terasaki and Petri diameter 65 mm

(provided with the microscope)

Holder for slide and Petri diameter 54 mm

(provided with the microscope)

M-793.4

Holder for 2+2 slides.

M-793.6

Holder for Utermöhl-Chamber (holder for Petri 54mm

needed)

Load-bearing side extension

(provided with the microscope)

③

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