Optika Italy B-1000 Series Manuale utente

Ver. 2.1 2020

Model

B-1000FL-LED

B-1000 Series

INSTRUCTION MANUAL

Page 2

Table of Contents

1. Warning 3

2. Symbols and conventions 3

3. Safety Information 3

4. Intended use 3

5. Instrument description 4

6. Unpacking 6

7. Assembling 6

7.1 Assembling the microscope 7

7.1.1 Manual version 7

7.1.2 Motorised version 9

8. Brighteld (transmitted light) observation procedures 10

9. Use of the microscope in Brighteld (transmitted light) 11

9.1 General switch on 11

9.2 Control keyboard 11

9.3 Brightness adjustment 11

9.4 Adjust the observation head 12

9.5 Adjust the interpupillary distance 12

9.6 Diopter adjustment 12

9.7 Use of eyeshields 12

9.8 Light path selection 13

9.9 Coarse focus tension adjustment 13

9.10 Focus stop lever 14

9.11 Stage 14

9.12 Centering the condenser 15

9.13 Effect of eld diaphragm 15

9.14 Aperture diaphragm 15

9.15 Use of oil immersion objective 16

9.16 Only for motorised version 17

9.16.1 Nosepiece rotation 17

9.16.2 Focusing 17

9.16.3 Stage 17

10. Fluorescence (reected light) observation procedures 18

11. Use of the microscope in Fluorescence (reected light) 19

11.1 LED switch on 19

11.2 Use of uorescence 19

11.3 Use of light excluding plate 20

12. Microphotography 21

12.1 Use of “C”mount camera 21

12.2 Use of Reex camera 21

13. Maintenance 22

14. Troubleshooting 23

Equipment disposal 25

Page 3

1. Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of

maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you

that this manual contains important information on safety and maintenance, and that it must therefore be made

accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses

that does not comply with this manual.

2. Symbols and conventions

The following chart is an illustrated glossary of the symbols used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed carefully.

ELECTRICAL SHOCK

This symbol indicates a risk of electrical shock.

3. Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the

operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety

regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility

to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure

safe operation of the unit.

4. Intended use

Standard models

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

IVD Models

Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the

subject.

Page 4

COARSE

FOCUSING

KNOB

NOSEPIECE

DIOPTRIC

ADJUSTMENT

RING

PHOTO/TV

PORT

MAIN SWITCH

EYEPIECE

FILTER

SELECTOR

LEVER

CONDENSER

CENTERING

KNOBS

TRANSMITTED

LIGHT BRIGHTNESS

ADJUSTMENT KNOB

FOCUS LOCK

LEVER

FINE FOCUSING

KNOB

OBJECTIVE

STAGE

FLUORESCENCE LED

5. Instrument description

CONTROL

KEYBOARD

Page 5

FIELD DIAPHRAGM

APERTURE

DIAPHRAGM

Opposite side

FLUORESCENCE

MAIN SWITCH /

BRIGHTNESS

ADJUSTMENT

KNOB

LIGHT PATH

SELECTOR

LEVER

TENSION

ADJUSTMENT

RING

CONDENSER

HEIGHT

ADJUSTMENT

KNOB

X-Y STAGE

MOVEMENT

KNOBS

Page 6

①

②

③

④

⑤

⑥

⑦

⑧

6. Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container

and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)

fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope

from the container and put it on a stable desk.

Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or

other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.

7. Assembling

Once opened the box, the microscope parts are the following:

① Frame

② Objectives

③ Stage

④ Condenser

⑤ Observationhead

⑥ Eyepieces

⑦ LEDuorescenceattachment

⑧ Lightexcludingplate

⑨ Dustcover

⑩ Allenwrench

⑪ Immersionoil(if100xobj.isincludedinthe

 conguration)

⑫Powersupply(2units)

⑪

⑩

⑨

⑫

Page 7

7.1 Assembling the microscope

7.1.1 Manual version

1. Put the microscope stand on a solid table. Insert

uorescence attachment above the stand, using

the Allen wrench to tighten the screw. (Fig. 1)

2. Insert the optical head above the attachment, us-

ing the Allen wrench to tighten the screw. (Fig. 2)

3. Insert eyepieces into the empty eyepiece sleeves.

(Fig. 3)

4. Insert the condenser under the stage: position

until it is well inserted into its holder (under the

condenser there is a pin that must fully enter the

holder guide). (Fig. 4)

5. Lock the condenser xing knob ①.

FFig. 1ig. 1

FFig. 2ig. 2

FFig. 4ig. 4

FFig. 3ig. 3

①

Page 8

FFig. 5ig. 5

FFig. 6ig. 6

FFig. 8ig. 8

FFig. 7ig. 7

6. Mount the stage: lower the support using the

coarse focus knob, then place the stage and

tighten the lock screw ①. (Fig. 5)

7. Screw each objective into the thread of the nose-

piece, clockwise with increasing magnication.

(Fig. 6)

8. Insert the power supply jack in the socket placed

in the rear side of the of the microscope: one for

transmitted light and one for uorescence. (Fig.

7-8)

①

Page 9

FFig. 9ig. 9

FFig. 10ig. 10

FFig. 11ig. 11

②

①

③ ④ ⑤⑥

7.1.2 Motorised version

1. Assemble the stage in the same way as the

manual version. Check the perfect alignment of

the rear part of the stage with the rear arm of the

frame. An imperfect alignment could lead to an

incorrect functioning of the system. (Fig. 9)

2. Connect the cable ① from the stage to the frame

and tight the locking screws of the connectors ②.

(Fig. 10)

3. Connect the provided cables: ③ 12V power sup-

ply for the motorised parts; ④6V microscope

power supply; ⑤ serial cable; ⑥ PS/2 mouse.

(Fig. 11)

• Connect power cables as the last step.

Page 10

8. Brighteld (transmitted light) observation procedures

Main switch

(Used commands) (Chapter)

Slide holder

Revolving nosepiece

Observation head

Diopter adjustment ring

Condenser centering

Aperture diaphragm

Field diaphragm

Revolving nosepiece

Light adjustment dial

Coarse and fine focusing knobs

Bring on “1” the main switch

Put a specimen on the stage

Insert 10X objective into the light path

Focus the specimen

Begin observation

Adjust field and aperture iris diaphragms

Insert desired objective for the observation and focus the

specimen

Adjust interpupillary distance

Adjust diopters

Center optical axis

Adjust light intensity

ON-OFF key on the keyboard

Light adjustment dial

Turn on the microscope and adjust light intensity 9

Fluorescence filter selector

Remove all fluorescence filter from the light path 11

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