Optika Italy B-1000FL-HBO Manuale utente

Model

B-1000FL HBO

B-1000 Series

INSTRUCTION MANUAL

Ver. 4.0 2019

Page 2

1. Warning 4

2. Symbols and conventions 4

3. Safety Information 4

4. Intended use 4

5. Overview 5

5.1 Manual version 5

5.2 Motorised version 7

6. Unpacking 8

7. Assembling 8

7.1 Manual version 8

7.2 Motorised version 9

7.3 Assembling the microscope 10

7.4 Only for motorised version 14

8. Brighteldobservationprocedures(transmittedlight) 15

9. UseofthemicroscopeinBrighteld(transmittedlight) 16

9.1 General switch on 16

9.2 Control keyboard 16

9.3 Brightness adjustment 16

9.4 Adjust the observation head 17

9.5 Adjust the interpupillary distance 17

9.6 Diopter adjustment 17

9.7 Use of eyeshields 18

9.8 Light path selection 18

9.9 Coarse focus tension adjustment 18

9.10 Focus stop lever 19

9.11 Stage 19

9.12 Condenser centering 20

9.13 Effectofelddiaphragm 20

9.14 Aperture diaphragm 20

9.14 Use of oil immersion objective 21

9.15 Only for motorised version 21

9.15.1 Nosepiece rotation 21

9.15.2 Focusing 22

9.15.3 Stage 22

10. Fluorescence observation procedures 23

11. Use of the microscope in Fluorescence 24

11.1 HBO bulb switch on 24

11.2 Centering of HBO bulb 24

11.3 Use of diaphragms 26

11.3.1 Field diaphragm 26

11.3.2 Aperture diaphragm 26

11.4 Use of the shutter 26

11.5 Useofuorescence 27

11.6 Use of light excluding plate 27

12. UseofcondenserforBrighteld/Darkeld/PhaseContrast(optional) 28

12.1 Brighteldobservation(BF) 28

12.2 Darkeldobservation(DF) 28

12.3 Phasecontrastobservation(PH) 29

12.4 Useofgreenlter 30

13. SimultaneousobservationinPhaseContrast+Fluorescence 30

14. DifferentialInterferenceContrastDICObservation(optional) 31

14.1 Koehler DIC transmitted light 31

14.2 Nomarski DIC transmitted light 32

15. Simultaneous Observation in Fluorescence and DIC 34

15.1 KoehlerDICreectedlight 34

15.2 NomarskiDICreectedlight 36

16. Microphotography 39

16.1 Use of “C”mount camera 39

16.2UseofReexcamera 39

Table of Contents

Page 3

17. Maintenance 40

18. Troubleshooting 41

Equipment disposal 43

Page 4

1. Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of

maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you

that this manual contains important information on safety and maintenance, and that it must therefore be made

accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use that

does not comply with this manual.

2. Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with caution.

ELECTRICAL SHOCK

This symbol indicates a risk of electrical shock.

3. Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the

operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety

regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility

to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure

safe operation of the unit.

4. Intended use

Standard models

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

IVD Models

Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the

subject.

Page 5

5. Overview

EYEPIECE

PHOTO / TV

PORT

OBSERVATION

HEAD

DIOPTER

ADJUSTMENT

RING

CONTROL

KEYBOARD

MAIN SWITCH

FOCUS LOCK

LEVER

INTENSITY

ADJUSTMENT

DIAL

FIELD

DIAPHRAGM

CONDENSER

STAGE

CONDENSER

HEIGHT

ADJUSTMENT

KNOB

NOSEPIECE

OBJECTIVES

FLUORESCENCE

POWER SUPPLY

UV SHIELD

5.1 Manual version

Page 6

FLUORESCENCE

FILTER

TURRET

COARSE

FOCUS KNOB

FINE

FOCUS

KNOB

TENSION

ADJUSTMENT

COLLAR

APERTURE

DIAPHRAGM

LIGHT PATH

SELECTOR

LEVER

DIC PRISM

SLOT

SLIDE HOLDER

X-Y STAGE

MOVEMENT

KNOBS

CONDENSER

CENTERING

SCREWS

HBO LAMP

HOUSING

SHUTTER

Opposite side

Page 7

5.2 Motorised version

Only the parts related to the motors are showed; all the other components of the microscope remain unchanged

with respect to the manual version.

NOSEPIECE

FOCUS

MOTOR

X AXIS MOVEMENT

KNOB

(MANUAL MOVEMENT)

STAGE

Opposite side

Y AXIS MOVEMENT

KNOB

(MANUAL MOVEMENT)

STAGE

CONNECTION

CABLE

NOSEPIECE

ROTATION

BUTTONS

Page 8

6. Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container

and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall

out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope from

the container and put it on a stable desk.

Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or

other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.

7. Assembling

Once opened the box, the microscope parts are the following:

7.1 Manual version

① Frame

② Fluorescence epi-illuminator

③ Objectives

④ Condenser

⑤ Observation head

⑥ Eyepieces

⑦ Stage

⑧ HBO lamp housing

⑨ Darkening plate

⑩ Microscope power supply

⑪ Fluorescence power supply

⑫ Power cord for uorescence power supply

⑬ Immersion oil (if 100x is included in the con-

guration)

⑭ Allen wrench

⑮ Dust cover

③

④

⑤

⑥

⑦

⑧

①

②

⑪

⑩

⑨

⑭⑬

⑮

⑫

Page 9

7.2 Motorised version

① Frame

② Fluorescence epi-illuminator

③ Objectives

④ Condenser

⑤ Observation head

⑥ Eyepieces

⑦ Stage

⑧ HBO lamp housing

⑨ Darkening plate

⑩ Microscope power supply

⑪ Fluorescence power supply

⑫ Motor power supply

⑬ Serial cable

⑭ PS/2 Mouse

⑮ Power cord for uorescence power supply

⑯ Immersion oil (if 100x is included in the con-

guration)

⑰ Allen wrench

⑱ Dust cover

③

④

⑤

⑥

⑦

⑧①

②

⑪

⑩

⑨

⑭ ⑬

⑮⑱⑰⑯

⑫

Page 10

7.3 Assembling the microscope

1. Insert the uorescence epi-illuminator over the

microscope and tighten the screw with the 2 mm

Allen wrench. (Fig. 1)

2. Screw the extension tube on the rear of the at-

tachment, using the 3 provided allen screws. (Fig.

3. Screw the lamp housing on the extension tube,

tightening the screws inside the holes. (Fig. 3)

4. Insert the optical head above the attachment,

using the 2 mm Allen wrench to tighten the screw.

(Fig. 4)

Fig. 2

Fig. 3

Fig. 4

Fig. 1

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