Optika Italy IM Series Manuale utente

Ver. 5.2 2021

Model

IM-3

IM-3F

IM-3FL4

IM-3LD2

IM-3LD4

IM-3LD4D

IM Series

INSTRUCTION MANUAL

Page 2

Table of Contents

1. Warning 4

2. Symbols and conventions 4

3. Safety Information 4

4. Intended use 4

5. Instrument description 5

5.1 IM-3 5

5.2 IM-3F 6

5.3 IM-3FL4 7

5.4 IM-3LD2 8

5.5 IM-3LD4 9

5.6 IM-3LD4D 10

6. Unpacking 12

6.1 IM-3 12

6.2 IM-3F / IM-3FL4 13

6.3 IM-3LD2 14

6.4 IM-3LD4 15

6.5 IM-3LD4D 16

7. Assembling 17

7.1 Installing the objectives 17

7.2 Installing stage extension or mechanical stage 17

7.3 Installing the stage insert 18

7.4 Installing the eyepieces 18

7.5 Installing the condenser 18

7.6 Installing the LED housing 19

7.7 Installingcolorlters 19

7.8 Installing the PC screen (IM-3LD4D) 20

7.9 Cable connection (IM-3LD4D) 20

7.10 Connecting the power supply 21

7.11 Installinguorescence 22

8. Brighteldobservation(transmittedlight) 26

9. Useofthemicroscopeinbrighteld(transmittedlight) 27

9.1 Turning on the microscope 27

9.2 Adjusting the light intensity 27

9.3 Adjusting the coarse focus tension 27

9.4 Diopter adjustment 27

9.5 Adjusting interpupillary distance 28

9.6 Use of eyeshields 28

9.7 Selecting the light path 28

9.8 Stage and stage inserts 29

9.8.1 Installing stage inserts 30

9.9 Aperture diaphragm 30

9.10 Usingcolorlters 31

10. Use of the microscope in phase contrast (transmitted light) 32

10.1 Installing the phase contrast slider 32

10.2 Phase contrast slider 32

10.3 Centering the phase ring 32

11. Use of the microscope in RPC (transmitted light) 34

11.1 Installing the RPC slider 34

11.2 RPC slider 34

11.3 RPC observation 35

12. Fluorescence observation (IM-3F / IM-3FL4) 36

13. Fluorescence observation (IM-3LD2 / IM-3LD4 / IM-3LD4D) 36

14. Use of the microscope inuorescence(reectedlight) 37

14.1 Centering the mercury bulb 37

14.2 Centeringtheelddiaphragm 39

14.3 Using the microscope 39

14.3.1Switchinguorescenceltercubes 39

14.3.2Availableuorescenceltercubes 40

14.4 Installinguorescencelter 41

Page 3

14.5 Use of the Anti-glow cap 42

14.6 Filter holder / Shutter 42

14.6.1InsertingaNDlter 42

14.6.2Usingthelterslider 43

15. Simultaneous observation in Phase Contrast + Fluorescence 44

15.1 IM-3F / IM-3FL4 44

15.2 IM-3LD2 / IM-3LD4 / IM-3LD4D 44

16. Microphotography 45

16.1 Use of C-mount cameras 45

16.2UseofReexcameras 45

17. Maintenance 46

18. Troubleshooting 47

Equipment disposal 49

Page 4

1. Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-

nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this

manual contains important information on safety and maintenance, and that it must therefore be made accessi-

ble to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does

not comply with this manual.

2. Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with caution.

ELECTRICAL SHOCK

This symbol indicates a risk of electrical shock.

3. Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the op-

eration voltage of the equipment and that the lamp switch is in off position. Users should observe all safety reg-

ulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility

to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure

safe operation of the unit.

4. Intended use

Standard models

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

IVD Models

Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the sub-

ject.

Page 5

FILTER HOLDER

PHASE

CONTRAST SLIDER

PHOTO/TV

PORT

DIOPTER

ADJUSTMENT

RING

EYEPIECE

LED

HOUSING

CONDENSER

COARSE

FOCUS KNOB

FINE FOCUS KNOB

TENSION

ADJUSTMENT

COLLAR

OBJECTIVE

STAGE

MICROSCOPE

BODY

LIGHT PATH

SELECTOR

LEVER

STAGE

PLATE

5. Instrument description

5.1 IM-3

MAIN SWITCH

Page 6

PHOTO/TV

PORT

DIOPTER

ADJUSTMENT

RING

EYEPIECE

PHASE CONTRAST SLIDER

LED

HOUSING

CONDENSER

FILTER HOLDER

COARSE

FOCUS KNOB

FINE FOCUS KNOB

FLUORESCENCE

POWER SUPPLY

TENSION

ADJUSTMENT

COLLAR

OBJECTIVE

STAGE

MICROSCOPE

BODY

LIGHT PATH

SELECTOR

LEVER

STAGE PLATE

FLUORESCENCE LAMP

HOUSING

UV SHIELD

5.2 IM-3F

FLUORESCENCE

CUBE LEVER

MAIN SWITCH

Page 7

5.3 IM-3FL4

PHOTO/TV

PORT

DIOPTER

ADJUSTMENT

RING

EYEPIECE

LED

HOUSING

CONDENSER

FILTER HOLDER

COARSE

FOCUS KNOB

FINE FOCUS

KNOB

FLUORESCENCE

POWER SUPPLY

MICROSCOPE

BODY

LIGHT PATH

SELECTOR

LEVER

STAGE

PLATE

FLUORESCENCE LAMP

HOUSING

UV SHIELD

STAGE

FLUORESCENCE

CUBE LEVER

TRANSMITTED LIGHT

ADJUSTMENT KNOB

Page 8

DIOPTER

ADJUSTMENT

RING

EYEPIECE

PHASE

CONTRAST

SLIDER

LED

HOUSING

FILTER HOLDER

COARSE

FOCUS KNOB

FINE FOCUS KNOB

OBJECTIVE

STAGE

MICROSCOPE

BODY

LIGHT PATH

SELECTOR

LEVER

STAGE PLATE

CONDENSER

PHOTO/TV

PORT

5.4 IM-3LD2

FLUORESCENCE

CUBE LEVER

TRANSMITTED LIGHT

ADJUSTMENT KNOB

REFLECTED LIGHT

ADJUSTMENT KNOB

Page 9

5.5 IM-3LD4

DIOPTER

ADJUSTMENT

RING

EYEPIECE

LED

HOUSING

FILTER HOLDER

COARSE

FOCUS KNOB

FINE FOCUS KNOB

OBJECTIVE

STAGE

MICROSCOPE

BODY

LIGHT PATH

SELECTOR

LEVER

STAGE PLATE

CONDENSER

PHOTO/TV

PORT

FLUORESCENCE

CUBE LEVER

TRANSMITTED LIGHT

ADJUSTMENT KNOB

REFLECTED LIGHT

ADJUSTMENT KNOB

X-Y MOVEMENT

KNOBS

Page 10

5.6 IM-3LD4D

DIOPTER

ADJUSTMENT

RING

EYEPIECE

FILTER HOLDER

COARSE

FOCUS KNOB

FINE FOCUS KNOB

OBJECTIVE

STAGE

MICROSCOPE

BODY

LIGHT PATH

SELECTOR

LEVER

STAGE PLATE

CONDENSER

FLUORESCENCE

CUBE LEVER

TRANSMITTED LIGHT

ADJUSTMENT KNOB

REFLECTED LIGHT

ADJUSTMENT KNOB

USB CAMERA

MONITOR

X-Y MOVEMENT

KNOBS

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