Optika Italy B-290 Series Manuale utente

Model

B-290 series (B-292 / B-292PLI / B-293 / B-293PLI)

B-290LD series (B-292LD1.50 / B-292LD1 / B-293LD1.50 / B-293LD1)

B-290TB series (B-290TB)

B-290 Series

INSTRUCTION MANUAL

Ver. 5.0 2020

Page 2

1. Warning 3

2. Symbols and conventions 3

3. Safety Information 3

4. Intended use 3

5. Overview 4

5.1 B-292 / B-292PLI / B-293 / B-293PL 4

5.2 B-292LD1.50 - B-292LD1 - B-293LD1.50 - B-293LD1 5

5.3 B-290TB 6

6. Unpacking 7

7. Assembling 7

7.1 B-292 / B-292PLI / B-293 / B-293PLI 7

7.2 B-292LD1 / B-292LD1.50 / B-293LD1 / B-293LD1.50 8

7.3 B-290TB 9

7.4 Assembling the microscope 10

7.4.1 B-292 / B-292PLI / B-293 / B-293PLI 10

7.4.3 B-290TB 12

7.5 Polarizing set (optional) 14

8. Use of the microscope 15

8.1 Switching on the microscope 15

8.2 Light intensity adjustment 15

8.3 Coarse focus tension adjustment 15

8.4 Stage 15

8.5 Adjust the interpupillary distance 16

8.6 Diopter adjustment 16

8.7 Use of oil immersion objective 16

8.8 Condenser centering 17

8.9 Aperture diaphragm 17

8.10 Useofuorescence 18

8.11 Use of the polarizer (optional) 18

9. Microphotography 19

9.1 Cameras with projection lens 19

9.2 Reexcamera 19

10. Use of software and digital head 20

11. Maintenance 21

12. Troubleshooting 22

Equipment disposal 23

Summary

Page 3

1. Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of

maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you

that this manual contains important information on safety and maintenance, and that it must therefore be made

accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses

that does not comply with this manual.

2. Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with caution.

ELECTRICAL SHOCK

This symbol indicates a risk of electrical shock.

3. Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the

operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety

regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility

to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure

safe operation of the unit.

4. Intended use

Standard models

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

IVD Models

Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the

subject.

Page 4

5. Overview

FINE FOCUS KNOB

PHOTO TUBE (ONLY FOR

TRINOCULAR HEAD)

INTENSITY ADJUSTMENT DIAL

STAGE

OBJECTIVES

COARSE FOCUS KNOB

CONDENSER

X/Y MOVEMENT

KNOBS

SLIDE HOLDER

CONDENSER

HEIGHT ADJUSTMENT

KNOB

CONDENSER

CENTERING SCREWS

OBSERVATION HEAD

-) BINOCULAR (B-292 / B-292PLI)

-) TRINOCULAR (B-293 / B-293PLI)

EYEPIECES

5.1 B-292 / B-292PLI / B-293 / B-293PL

APERTURE DIAPHRAGM

Page 5

INTENSITY ADJUSTMENT DIAL

COARSE FOCUS KNOB

FINE FOCUS KNOB

CONDENSER

SLIDE HOLDER

CONDENSER

HEIGHT ADJUSTMENT

KNOB

STAGE

OBJECTIVES

X/Y MOVEMENT

KNOBS

CONDENSER

CENTERING SCREWS

EYEPIECES

PHOTO TUBE (ONLY FOR

TRINOCULAR HEAD)

OBSERVATION HEAD

-) BINOCULAR

(B-292LD1 / B-292LD1.50)

-) TRINOCULAR

(B-293LD1 / B-293LD1.50)

FLUORESCENCE

ATTACHMENT

5.2 B-292LD1.50 - B-292LD1 - B-293LD1.50 - B-293LD1

APERTURE DIAPHRAGM

Page 6

INTENSITY ADJUSTMENT DIAL

COARSE FOCUS

KNOB

FINE FOCUS KNOB

STAGE

X/Y MOVEMENT

KNOBS

SLIDE HOLDER OBJECTIVES

DIGITAL HEAD

TABLET

EYEPIECES

CONDENSER

HEIGHT ADJUSTMENT

KNOB

CONDENSER

CENTERING SCREWS

5.3 B-290TB

APERTURE DIAPHRAGM

Page 7

6. Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container

and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)

fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope

from the container and put it on a stable desk.

Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other

residuals may deteriorate the nal image quality and corrode the optics surface in a short time.

7. Assembling

Once opened the box, the microscope parts are the following:

7.1 B-292 / B-292PLI / B-293 / B-293PLI

① Frame

② Observation head

binocular (B-292 / B-292PLI)

trinocular (B-293 / B-293PLI)

③ Photo tube (only B-293 series)

④ Eyepieces

⑤ Objectives (4X / 10X / 40X / 100X)

⑥ Dust cover

⑦ Green lter

⑧ Power supply

⑨ Immersion oil

⑩ Tension adjustment tool

③

④

⑤

⑥

⑦

⑧

①

②

⑨

⑩

Page 8

7.2 B-292LD1 / B-292LD1.50 / B-293LD1 / B-293LD1.50

① Frame

② Observation head

binocular (B-292LD1 / B-292LD1.50)

trinocular (B-293 / B-293PLI)

③ Photo tube (only B-293 series)

④ Eyepieces

⑤ Objectives

10X/20X/40X/50X: B-292LD1.50 - B-293LD1.50

10X/20X/40X/100X(dry): B-292LD1 - B-293LD1

⑥ Dust cover

⑦ Fluorescence attachment

⑧ Power supply

⑨ Tension adjustment tool

③

④

⑤

⑥

⑦

⑧

①

②

⑨

Page 9

7.3 B-290TB

① Frame

② Digital observation head

③ Eyepieces

④ Objectives (4X / 10X / 40X / 100X)

⑤ Dust cover

⑥ Green lter

⑦ Immersion oil

⑧ Power supply

⑨ Tension adjustment tool

⑩ Tablet power supply

⑪ OTG cable

⑫USB cable

⑬ Touch pen

⑭ Tablet + keyboard

②

⑩

⑪

⑫

⑬

⑭

③

④

⑤

⑥

⑦

⑧

①

⑨

Page 10

7.4 Assembling the microscope

7.4.1 B-292 / B-292PLI / B-293 / B-293PLI

1. Remove the dust cap from the microscope frame

and from the bottom of the observation head.

2. Insert the optical head above the stand and tight-

en the screw. (Fig. 1)

• Hold the head with one hand during the

locking in order to avoid that the head falls.

3. Insert both eyepieces into the tubes of the optical

head. (Fig. 2)

4. Insert the power supply jack in the socket placed

at the rear side of the microscope. (Fig. 3)

Only for trinocular head

5. Unscrew the protection cap mounted on the

photo port and screw the photo tube. (Fig. 4)

FFig. 1ig. 1

FFig. 2ig. 2

FFig. 3ig. 3

FFig. 4ig. 4

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